自己放射法에 依한 白鼠舌下腺細胞의 蛋白合成과 移動
SYNTHESIS AND MIGRATION OF PROTEINS IN THE SUBLINGUAL GLAND OF MICE BY RADIOAUTOGRAPHY
조용명,
소속 상세정보
조용명 ( ) - 서울대학교 치과대학 구강해부학교실
KMID : 0362619890100090587
Abstract
Twenty-nine male mice weighing approximately 20 grams were utilized in this study. They were received a single intraperitoneal injection of leucine-H8. The dose, was
defined by a radioactivity content of Z. 5/tc for each gram of body weight.
This procedure was carried out 10 minutes after injection in the first group.
Whereas the animals of the remaining 5 groups, were sacrificed 30 minutes, 1 hour,
2 hours. 6 hours and 24 hours after injection respectively.
The sublingual gland was fixed for 3 hours in paraformaldehyde and routinely processed the histology.
Sections 6 p thick were stained with toluidine blue 0 and radioatitographed by the dipping technique. The autoradiographs were exposed for 15 days,
The present study was undertaken to determine the turnover time for the synthesis and migration of proteins in the sublingual gland of mice.
The results permited the following conclusions;
1. Thirty minutes after leucine-119 injection, silver grains were locat: d in the ergastoplasm.
2. One hour after leucine-H8 injection, the silver grains were observed in the proximal zymogen region. The distal zymogen region were reached maximum specific activity 6 hours after leueine-H´ injection
3. Six hours after leucine-Hg injection, labeled proteins were shown in the excretory
ducts.
4. The synthesis and migration of proteins of the sublingual acinear cells were slower than that of the pancreatic acinar cells.
키워드
원문 및 링크아웃 정보
등재저널 정보